FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679045
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC658853.9239567730465827Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG98360.5858091951079334No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA72560.4321504188392807No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT59920.3568695300006849No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT56310.3353692128561176No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA48260.28742529235368913No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT29850.17777963068291797No Hit
TGTGTCGCTGGTGCGGTCTCAAATATATTTTGCCTCAATCAACACAAGAT26810.15967410045591393No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA19720.11744771581464464No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)