FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362966
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1069867.8494988136167745Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT36420.2672113611051192No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG28260.20734192929244016No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA21850.16031214278272532No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA16810.12333396431018823No Hit
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG16110.11819810618900252No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA13880.10183672960293946No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA13760.10095629678216478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)