FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746348
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGGCATCTCGTA1380897.907301408424895TruSeq Adapter, Index 9 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGGCATATCGTA40970.23460387047713285TruSeq Adapter, Index 9 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGGCATCCCGTA29810.17069908174086723TruSeq Adapter, Index 9 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGGCATCTCGCA22690.12992828462597375TruSeq Adapter, Index 9 (97% over 39bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT20310.11629984401734363No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGGCATCTCGGA20020.11463923570788868TruSeq Adapter, Index 9 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACAGGCATTTCGTA18330.10496189762865134TruSeq Adapter, Index 9 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)