FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001809144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3365719
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2618747.780625774165936Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT117170.34812769574643637No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG83040.2467229141826754No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA72110.2142484265620511No Hit
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG65170.19362876104630244No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA47310.1405643192435257No Hit
CTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC46560.13833596922381222No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT44870.13331475384605787No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA42300.1256789411118397No Hit
CTCCTCACACGCCATGAACTGGGAAACCTGCAATACACACAGAGCATGGG40320.11979609705979614No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA38870.11548795368835009No Hit
TGTGTCGCTGGTGCGGTCTCAACCGGTGTGATTGGCGATACAAGATCGGA36230.10764416161895868No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT34690.10306861624514703No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT33750.10027575088710614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)