FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001809149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378762
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCGTCATCTCGTA35287710.443973265947704TruSeq Adapter, Index 27 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT82550.24432025694618326No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG63460.18782027263240206No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA48010.142093465002862No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA34130.101013329734382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)