FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588506
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATCTCGTA566113.5637888682825247TruSeq Adapter, Index 5 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAACATCGATATCGTA65240.41070036877418153TruSeq Adapter, Index 5 (97% over 36bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG54780.3448523329468066No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT35090.2208993859639183No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA29220.1839464251315387No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT19950.12558970504360703No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA16060.10110128636593126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)