FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085657
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC12472611.488527223607456Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG24470.22539347141868932No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA11800.10868994535106391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)