FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478935
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATCTCGTA1393359.421306548293197TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGATGAATATCGTA71180.4812922812699679TruSeq Adapter, Index 7 (97% over 36bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT18990.1284032090659833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)