FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949405
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATCTCGTA11762812.38965457312738TruSeq Adapter, Index 18 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT28090.2958695182772368No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG19510.2054971271480559No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCTGTAATATCGTA17930.18885512505200627TruSeq Adapter, Index 18 (97% over 37bp)
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA15360.16178553936412807No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA10620.11185953307597918No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT10130.10669840584365997No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)