FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949405
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC12252212.905135321596159Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT27540.29007641628177644No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG18940.19949336689821523No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA15230.16041626071065562No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA10440.10996360878655578No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT10210.10754103886118148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)