FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820629
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTA529226.448955618190437TruSeq Adapter, Index 7 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT24660.30050120090808397No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG17200.2095953226122889No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATATCGTA16710.20362429307275273TruSeq Adapter, Index 7 (97% over 37bp)
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA12330.15025060045404198No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA10920.13306865830966247No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT9330.11369327674259622No Hit
ACATGATAAATGGGCCTGTGACAAGGTAGGGATTATCATCCAACTGACAA8920.10869710916869865No Hit
CTCAGTACCTAACTGCCAATCAACACACTGCCAATGCGGGTGGGCTAAGC8920.10869710916869865No Hit
CTCGTTGCCTACTGGGAGAAGACTTTCAAGATTGACCTATTCAGGCCACA8630.10516323454325888No Hit
CTCGTTCTTGTAGCAGAAGGAGTTGGCCCTGGGCAGCAGGAACTTGTTCT8600.10479766130614444No Hit
CTGGGGGACGAGGCCATGGAGCGCTTCCGCGAGGATGAGGGCTTCATTAA8590.10467580356043962No Hit
CTGGACGCCGACGATGCCGAAGATGAAGAAGACGAAGAAGCAGAGCAGCA8550.10418837257762034No Hit
TCATGGTCTCTGCTTACTACTTGATTCTCTCCTGCATGTTTTTGATGCCT8490.10345722610339142No Hit
CTCGTTCTCATAGCAGAAGGAGTTGGCACTGGGCAGCAGGAACTTGTTCT8470.10321351061198178No Hit
CCGTGGGGCTGGAAGTCCTGAGAATAGGAATGATGGACATTTATCTGGTC8240.10041078246077095No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)