FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820629
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC561956.84779601988231Illumina Single End PCR Primer 1 (100% over 50bp)
CTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC24960.3041569332792285No Hit
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG23690.28868099957471643No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT23600.2875842798633731No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG16250.19801883677033105No Hit
CTCCTCACACGCCATGAACTGGGAAACCTGCAATACACACAGAGCATGGG14290.1741347186121865No Hit
CTCCATAGGGAAGCCTGTGTCTCCTGCCCCCAGGGCGCACCCTCAGTGCA13100.15963364687331302No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA12070.14708229906571665No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA10460.1274632020072408No Hit
CTCGTGCTCTGGGGCCAAAGCCCAGTAAACCAAGTTAACAAACCAAGCCA10430.12709762877012631No Hit
CTCCAGCCTCAAACAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGG9960.12137031472199983No Hit
TGGTGGCCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGT9570.11661786263951189No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT9230.11247469928554804No Hit
CTGGCTGGCTCAAGGATGGGCAGCGTCCATCTGCTGCAGGCATCCTGGCG8910.10857525142299383No Hit
CTGCTGTCACCTATTTCTTTGACCTCCACCAACCCTGGGAAATTGCTGCT8760.10674738523742154No Hit
CTCTCCGGATACTCGAAGATAAGCCACACCTGGCGCTGGAACTCGTTGCG8550.10418837257762034No Hit
CTGTGACATTCACCACCCCAATCTGTGGCCTGAATAGGTCAATCTTGAAA8540.10406651483191552No Hit
GCATGCGCATCCTTGTCACGTTGCTGCTGGATACGCTGCCCATGCTGGGC8380.10211679090063842No Hit
CTGGGAGGTGCAGAACAGGACAGACCAGCGGGGTGGCATAGAGAAAGTCC8360.10187307540922878No Hit
CTCCAAGGACCGCAGACTTCTGCTGAAGTGTACCGCATCCTCAAACAGAA8250.10053264020647576No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)