FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365132
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA1074957.874330101411439TruSeq Adapter, Index 10 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT37910.2777020830220081No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG27370.20049343213696552No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATATCGTA25810.18906596578206356TruSeq Adapter, Index 10 (97% over 38bp)
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA24780.1815209078682501No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA16930.12401731114646788No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA15230.11156430293920296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)