FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1317766
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTTACATCTCGTA13238110.0458655026765TruSeq Adapter, Index 7 (97% over 35bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTTACATCTCGTA43830.3326083690124043TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTTACATATCGTA16990.1289303260214636TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)