FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477271
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC321692.177596392266551Illumina Single End PCR Primer 1 (100% over 50bp)
CTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC59090.3999943138395054No Hit
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG32590.22060948871263295No Hit
TGGTGGCCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGT26410.1787755936453095No Hit
CTGGCTGGCTCAAGGATGGGCAGCGTCCATCTGCTGCAGGCATCCTGGCG24860.16828327368505847No Hit
ACATGCTCACCCACACACACGCTCACACATACACTCACACACACCTGCTC24290.1644248076351597No Hit
CTCTCCGGATACTCGAAGATAAGCCACACCTGGCGCTGGAACTCGTTGCG21300.14418478396990125No Hit
GCATGCGCATCCTTGTCACGTTGCTGCTGGATACGCTGCCCATGCTGGGC20310.13748323767270867No Hit
AGATGCTGCTCAAATGGGTGGCCTACGGCTTTAAGGTGTACTTCACCAAC20090.1359940051622214No Hit
CTGGGAGGTGCAGAACAGGACAGACCAGCGGGGTGGCATAGAGAAAGTCC19750.13369246400965024No Hit
CTCCTCACACGCCATGAACTGGGAAACCTGCAATACACACAGAGCATGGG19560.13240630865968397No Hit
ACATGCTCACACAATTACATACACCGACTCACGCACACATGCTCACACAA19540.1322709238860033No Hit
CTGCTGCTTACCATAAACTATGAAAAGCACAGGTTTTAAGCCCGCCAGGA19480.13186476956496135No Hit
CTCCAAGGACCGCAGACTTCTGCTGAAGTGTACCGCATCCTCAAACAGAA19320.13078169137551607No Hit
ACATGCTCACACACAAGCACACATGATCACACAGGCACACATGATCACAC19230.1301724598939531No Hit
CTGCTCAGCCCGAAGCAGGGCTCACACTTTTGAAGGTGGTTTCTCCTCTG19120.1294278436387095No Hit
CTTGCCATGTACAAGGGGGACCTGTTTCAGATATTCCATGGAGACCCTGG19000.12861553499662554No Hit
CTCCAGCCTCAAACAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGG18920.1280739959019029No Hit
CTGTGACATTCACCACCCCAATCTGTGGCCTGAATAGGTCAATCTTGAAA18870.12773553396770126No Hit
CTGTTTTCAGCCTGGTTCATTTGGTACAAGTGACTCAGAAAATGGCCTTT18780.1271263024861383No Hit
CTGATCTCCAAGGTCCTTCCTAAGAATCTGGGATCTGGAGGATCCCAGGG18710.12665245577825598No Hit
CTGCCCTCCTGTTTCGATTACGTGATGCACTTTCTGACTGTGTTCTGGAA18450.12489245372040741No Hit
ACATGCTCACACACGATTACATACACCTCCCACATACACGATTACATGCA18310.12394476030464283No Hit
CTGTGCCTCCGAACTGGGCTTTCTTCTCTTCTCCCTCACCATGGCCATCA18180.12306475927571854No Hit
TGATGTTCTTTGCCCCTTCTACCAGGCCCCCTCCAAACACTGTGCTCCAG18130.1227262973415169No Hit
GTGTGTCCATCCTTTGGTAACATTTCCTTTTTCTTTTTCAGCCACCGGAT18120.12265860495467655No Hit
GTGTGCCATCTGTGTTTGTGGGTGTCAGGGCAGGGGACAGAATCAGGGTC18030.12204937347311359No Hit
CTGTTCTTACCTACAATGGAGAGAATCACCACCACAAAGTCAAAGATGTT17760.12022167902842472No Hit
CTGGGGGCAGCATTTCTGGAATCCTCCTAACCATTGTTCACTGCTTGTAG17440.11805552264953419No Hit
GGATGGCAGGGGACAAGGGGGGCCCGTGGCCCTCCACAGTGCTGACTGTG17130.11595705865748397No Hit
CGATGGTTGTGGTGTGGGGCTGAGGAGGAAGAGCGGTGCTGGGATTTCTT16880.11426474898647573No Hit
CTTGTTCTGCTTGTCTTCATGTCGCATCTCCTCCTCTGGGGGCTGGAAGT16860.11412936421279508No Hit
CTAGAGGACCAGGGGAGCCTACAGGAGAGGTGCCAGGGGGCAAAGAGAGA16810.11379090227859343No Hit
TCGTGGGCTTCGTCATCGTCACCTTTCAGGAGCAGGGGGAGCAGGAGTAC16580.11223397738126586No Hit
CTGATTGCCCCTGGGCCCTAGTGAGAACACTCCAGCCTGGATATGTTCAG16500.11169243828654324No Hit
CTCTAGGCTTCTGTAAAAACCTCCCCCACTGCATGGATTTGGCTGTTTAA16320.1104739753234173No Hit
CTGGGATTACCATTGCTCTGGGACCATCTTCTGAGTCAGACTTGGGGAGC16310.11040628293657696No Hit
CTCCTATCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC16170.10945858952081237No Hit
CTGCTGTCACCTATTTCTTTGACCTCCACCAACCCTGGGAAATTGCTGCT16000.10830781894452678No Hit
CTCCCAACTATTTGCCGGGAAGACCCAGAGATACATGGCTATTTCAGGGA15820.10708935598140083No Hit
CTTCAGCGTCTCAACCACCATGGGGCTCATCTCACCTGCCATGGTGAAGA15790.10688627882087984No Hit
ACATGCTCCCACATGCTCACCTATACACATGCTCACATACACACGAGTAC15650.10593858540511525No Hit
CTTGTACCCAGGAAGAGACATAGGCGAGAGGGGAGCAGTTGGTGAGGAGC15540.10519396914987161No Hit
CTCTGAGCATCTTCCCCACCATCATCCTCCAAGTCATCAAGATCAGATTC15300.10356935186570371No Hit
CTGGTGGTGGAGACGCTGATGTCCTCACTGAAACCCATCGGCAACATTGT15210.10296012038414075No Hit
CTCATGCTGGCGTCGTCAAGGAGCAATCCACTGCTTGCTGCTCTGTCTCT15080.10208011935521648No Hit
CTGCTGCACACACAGACAGGCCCTCTCCCTCTACCAGACAACACCGCCAG15060.10194473458153583No Hit
CTCCTTCTGGCCTGTAGGCTCCCATTGGGGGTGGTTTGGGTTTTCTCAAG15040.10180934980785515No Hit
CTGTTTGGCGGCAAGTTTAATTTTGATGAAACGCAAACCAAGCGGAGCAC15020.10167396503417452No Hit
CTGGCTGGCCCCAGGTAAGTGTACTTGCTTTAGGGCAGGCTCAGGGGTTC14970.10133550309997284No Hit
CTAGGCAGTAGTGAAAGAGGGGTACCCTTCCCAGAACTGAACTCACTTTC14880.10072627161840988No Hit
CTCAAGACAGATGCCGGACAGGGCTGCTGCTCCGAAGGAAAAGGCTCCCA14840.10045550207104857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)