FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260082
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC718345.700740110564233Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT14870.11800819311759075No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG14770.11721459397086856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)