FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1385098
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATCTCGTA1139048.2235336416629TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAACAAATATCGTA78160.5642922017070272TruSeq Adapter, Index 10 (97% over 36bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT24710.17839892917324263No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG16190.11688703615195459No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA13880.10020951586097158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)