FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065497
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATCTCGTA442424.1522406914331995TruSeq Adapter, Index 14 (97% over 38bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG20310.1906152715587186No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGCAATATCGTA15090.14162404962191352TruSeq Adapter, Index 14 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT11310.10614764752974433No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA10990.10314435423093636No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)