FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869640
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGTCAATCTCGTA712993.8135149012644143TruSeq Adapter, Index 18 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGTCAATATCGTA77200.41291371600949917TruSeq Adapter, Index 18 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT45050.24095547805994738No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG35670.19078539184014034No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA23930.127992554716416No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA20770.11109090520100126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)