FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600128
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC675344.220537357011439Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG41190.2574169066474682No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT25270.15792486601071914No Hit
AGATCGGATGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC23590.14742570594352453Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA22840.14273858091352692No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT18140.11336593072554196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)