FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688799
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTA141322.0516870669092144TruSeq Adapter, Index 6 (97% over 37bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTA47210.685395884721087TruSeq Adapter, Index 6 (97% over 37bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTA14930.2167540893642412TruSeq Adapter, Index 6 (97% over 37bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTA11220.1628922225496843TruSeq Adapter, Index 6 (97% over 37bp)
ACATGATAAATGGGCCTGTGACAAGGTAGGGATTATCATCCAACTGACAA8210.11919297211523244No Hit
AGATGACCAAGACGAAGAAGATCATGTATGTTTTCCCGGCTGCTCGTAAA7000.10162616380105083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)