FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986831
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGTATCTCGTA1869969.411771811492773TruSeq Adapter, Index 9 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGCATCTCGTA107940.5432772087812199TruSeq Adapter, Index 9 (97% over 38bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG100640.5065352815614413No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA47750.24033246914307257No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT43770.22030056909722065No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT33780.17001949335398936No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA30200.1520008495941527No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATCAAGAATCTCGTA26520.13347889176281222TruSeq Adapter, Index 9 (97% over 38bp)
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT20980.10559529220150077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)