FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362197
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTA827366.073717678133192TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG99240.7285289866296872No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT86660.6361781739351944No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA72210.5300995377320608No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATATCGTA47150.34613202055209347TruSeq Adapter, Index 7 (97% over 35bp)
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT46270.33967186831273305No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA42000.3083254477876548No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT37890.2781536003970057No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA27200.1996774328529574No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT23400.1717813209102648No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT22660.16634892016352992No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA21940.16106334105859874No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT19800.1453534253856087No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA14790.10857460411379558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)