FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429031
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC855415.985944321711705Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT45360.31741788666585957No Hit
CTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC41220.28844720653365813No Hit
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG34080.2384832799288469No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG33610.23519433798147135No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA27230.1905487004830546No Hit
CTCCTCACACGCCATGAACTGGGAAACCTGCAATACACACAGAGCATGGG22910.16031842556249656No Hit
CTCCATAGGGAAGCCTGTGTCTCCTGCCCCCAGGGCGCACCCTCAGTGCA21250.14870216251431914No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA20550.14380373833737686No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT19970.13974504401933896No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA17300.12106105465871629No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA16410.11483305820517539No Hit
CTCCAGCCTCAAACAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGG15980.111824026210768No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT15840.11084434137537953No Hit
TGGTGGCCCAGTCCATCGTGGGCTGCATCATCGACTCCTTCATGATTGGT15490.1083951292869084No Hit
CTCGTGCTCTGGGGCCAAAGCCCAGTAAACCAAGTTAACAAACCAAGCCA15150.1060158946866793No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA14950.10461634492183863No Hit
CTGGGAGGTGCAGAACAGGACAGACCAGCGGGGTGGCATAGAGAAAGTCC14620.10230708780985157No Hit
GCATGCGCATCCTTGTCACGTTGCTGCTGGATACGCTGCCCATGCTGGGC14430.10097751553325295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)