FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974078
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTA21703210.994094458273686TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATATCGTA127390.6453139136346183TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT46940.23778189109042294No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG33930.17187770695990737No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA26830.13591154959429164No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATTTCGTA24040.12177836944639471TruSeq Adapter, Index 6 (97% over 36bp)
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA19850.10055327094471445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)