FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974078
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC21711410.998248296166615Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGGGTGTAGATCTCGGTGGTCGCC73250.3710592995818808Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT43970.2227368928684682No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG31310.15860568832639846No Hit
CTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC28430.14401659914147263No Hit
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG27720.14041998340491105No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA26120.13231493385773002No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)