FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618955
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGGTCATCTCGTA17979911.105867673900757TruSeq Adapter, Index 2 (97% over 36bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGGTCATCTCGTA61420.37938052632716784TruSeq Adapter, Index 12 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGGTCATATCGTA35140.21705359321290588TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGGCCATCTCGTA30430.18796075246069222TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAAGGACATCTCGTA19940.12316586934164322TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)