FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001809239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896608
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATCTCGTA2242667.742366243551077TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTAATCCATATCGTA94170.32510439797169655TruSeq Adapter, Index 22 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT51940.17931318286768522No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG43050.14862211248467172No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)