FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888882
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATCTCGTA20199910.69410370790764TruSeq Adapter, Index 1 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG156510.8285853748407788No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT124190.6574788684523438No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA105110.55646673534927No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATATCGTA82890.43883101220722104TruSeq Adapter, Index 1 (97% over 37bp)
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT75890.40177205352160694No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA64060.3391424133429193No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT57920.30663641243868067No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT41810.22134786609221752No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA39450.20885370287821048No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT32480.1719535683012491No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA30950.1638535387599649No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT27930.14786524515559998No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAATGAATTTCGTA20440.10821215936199297TruSeq Adapter, Index 1 (97% over 37bp)
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA19370.1025474328200491No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)