FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657876
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATCTCGTA994015.995683633757893TruSeq Adapter, Index 7 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATATCGTA132900.8016281072890855TruSeq Adapter, Index 7 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG95180.5741080756341247No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT78920.4760307767287783No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA72310.4361604848613527No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT54740.3301815093529311No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA43030.2595489650613194No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT38860.23439629984389662No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA27630.16665902636867896No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT26640.16068753030986635No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT20700.12485855395699076No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA19930.12021405702235873No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAGCCAATTTCGTA19320.11653465035985804TruSeq Adapter, Index 7 (97% over 37bp)
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT18290.1103218817330126No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)