FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657876
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1097186.6179859048565755Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG84010.5067327110109562No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT69370.4184269511109395No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA65560.39544573900581226No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT51530.31081938576829626No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA39870.24048843218672566No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT35110.2117769965908186No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA25980.15670653293732462No Hit
TGTGTCGCTGGTGCGGTCTCAAATATATTTTGCCTCAATCAACACAAGAT24020.14488417710371584No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA18290.1103218817330126No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT18040.1088139281828074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)