FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617090
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTA20339312.577716762827054TruSeq Adapter, Index 7 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG146810.9078653630904897No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA106850.6607548126573042No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT90730.5610695755956687No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA70700.43720510299364906No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT63470.3924951610609181No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT42040.25997316166694495No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT41050.25385105343549214No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATATCGTA39800.24612111879981943TruSeq Adapter, Index 7 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGCAATCTCGTA34110.2109344563382372TruSeq Adapter, Index 7 (97% over 37bp)
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA24960.1543513348051129No Hit
AAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTATGCCGTC20930.12943002553970404TruSeq Adapter, Index 7 (96% over 31bp)
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA19460.12033962240815292No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT17020.10525078999931976No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)