FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001809267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences714765
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTA74051.0360048407518556TruSeq Adapter, Index 7 (97% over 35bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTA24810.34710709114184385TruSeq Adapter, Index 7 (97% over 35bp)
ACATGATAAATGGGCCTGTGACAAGGTAGGGATTATCATCCAACTGACAA8840.12367701272446188No Hit
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCTGAATCTCGTA8490.11878029841976033TruSeq Adapter, Index 9 (97% over 35bp)
AGATGACCAAGACGAAGAAGATCATGTATGTTTTCCCGGCTGCTCGTAAA7220.10101222079984329No Hit

[FAIL]Adapter Content

Adapter graph