FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001809273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332273
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATCTCGTA777955.839268678416511TruSeq Adapter, Index 11 (97% over 38bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG90530.6795153846096108No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA62570.46964848796005026No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT48220.3619378310601506No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT43190.3241828063767711No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA40350.3028658540704495No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT25890.194329540567136No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT24140.18119409460373362No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTAGTAATATCGTA20530.15409754607351497TruSeq Adapter, Index 11 (97% over 38bp)
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA14320.10748547782624132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)