FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227843
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACGGAATCTCGTA419743.418515233625146TruSeq Adapter, Index 7 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT53980.4396327543505155No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG45790.37293041537069477No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA27670.22535454451424164No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA22330.1818636421757505No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT21990.17909455850625852No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA19370.15775632552370294No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT18680.15213671454738106No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA18610.15156660908601507No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA16760.13649953617848534No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT15940.1298211579167695No Hit
TGTATCGCCAATCACACCGGTTGAGACCGCACCAGCGACACAAGATCGGA15910.1295768270047555No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT13830.11263655043845182No Hit
TGTGGAGATGGTCGCATTGCTGAGACCGCACCAGCGACACAAGATCGGAA12620.102781870320554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)