FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262073
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTA679895.38708933635376TruSeq Adapter, Index 2 (97% over 36bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT63840.5058344485620087No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG55360.4386434065224436No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA32020.2537095714748671No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA29660.23501017769970517No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT24900.19729445127183612No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT23910.1894502140525944No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA23190.18374531425678228No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATATCGTA23040.18255679346598808TruSeq Adapter, Index 2 (97% over 36bp)
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA21370.1693245953284794No Hit
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA21360.16924536060909315No Hit
TGTATCGCCAATCACACCGGTTGAGACCGCACCAGCGACACAAGATCGGA20090.15918255124703565No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT18320.14515800591566413No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT17500.13866075892598922No Hit
CTGTGTAGCAGAAGACAGGGCATGAGACCGCACCAGCGACACAGAGATCG17390.13778917701274015No Hit
TGTGGAGATGGTCGCATTGCTGAGACCGCACCAGCGACACAAGATCGGAA16090.1274886634925238No Hit
TGTGGCAAACCACTGGTGTCTGAGACCGCACCAGCGACACAAGATCGGAA14980.11869360964064679No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)