FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001809292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262073
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC714325.659894475200721Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT61600.48808587141948206No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG52820.4185177877983286No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA31380.24863854943414526No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA28790.22811675711309887No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT23940.18968791821075326No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT23780.18842016270057277No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA22560.17875352693544666No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA21100.16718525790504987No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA20850.16520438992039288No Hit
TGTGTCGCTGGTGCGGTCTCAACCGGTGTGATTGGCGATACAAGATCGGA19490.15442846808385885No Hit
TGTGTCGCTGGTGCGGTCTCATTTGTTTTTGTTTCCACTTGACACAAGAT17820.14119626994635018No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT16790.13303509384956339No Hit
CTGTGTCGCTGGTGCGGTCTCATGCCCTGTCTTCTGCTACACAGAGATCG16630.1317673383393829No Hit
TGTGTCGCTGGTGCGGTCTCAGACACCAGTGGTTTGCCACAAGATCGGAA14380.11393952647747001No Hit
TGTGTCGCTGGTGCGGTCTCAGCAATGCGACCATCTCCACAAGATCGGAA13230.10482753374804785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)