FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434006
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC852245.943071367902227Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG108320.7553664350079428No Hit
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT77490.5403743080572885No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA76490.5334008365376435No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT56620.394837957442298No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA49220.34323426819692526No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT40070.2794270037921738No Hit
TGTGTCGCTGGTGCGGTCTCAAATATATTTTGCCTCAATCAACACAAGAT27730.19337436523975493No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA27470.19156126264464723No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA22640.15787939520476205No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT20640.1439324521654721No Hit
TGTGTCGCTGGTGCGGTCTCATTTGTTTTTGTTTCCACTTGACACAAGAT19130.1334025101708082No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA14530.10132454118044136No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)