FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175666
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATCTCGTA453833.8601949873518495TruSeq Adapter, Index 16 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT18530.15761279138803028No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG14980.12741714058244435No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAGAATATCGTA12080.10275027091027555TruSeq Adapter, Index 16 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)