FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175666
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC478664.071394426648385Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT17880.15208401025461313No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG14430.12273894116186058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)