FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131783
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC903647.984216055551285Illumina Single End PCR Primer 1 (100% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT47640.4209287469417724No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG35360.3124273822808789No Hit
TGTGTCGCTGGTGCGGTCTCACGGTAACTGGCCTCGGCACAAGATCGGAA27300.24121231720214917No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA20160.17812601885697169No Hit
TGTGTCGCTGGTGCGGTCTCATGAGGCAGACAAGCAGCACAAGATCGGAA19010.16796506044003134No Hit
TGTGTCGCTGGTGCGGTCTCAGATAGGCAATGATTACTTCGACACAAGAT18960.1675232796392948No Hit
TGTGTCGCTGGTGCGGTCTCAGCACCCAACTCAGCATCACAAGATCGGAA16700.14755478744600334No Hit
TGTGTCGCTGGTGCGGTCTCAACCGGTGTGATTGGCGATACAAGATCGGA15790.13951437687259838No Hit
TGTGTCGCTGGTGCGGTCTCATAACTGTCATCTTACCATCAGCACAAGAT15660.13836574679068336No Hit
TGTGTCGCTGGTGCGGTCTCACTCCCTACTCGGGCCACACAAGATCGGAA15410.1361568427870007No Hit
TGTGTCGCTGGTGCGGTCTCAGCGGTAAAACCAGTATTTCGTCACAAGAT14320.12652602133094418No Hit
TGTGTCGCTGGTGCGGTCTCATTTGTTTTTGTTTCCACTTGACACAAGAT12500.11044520018413423No Hit
TGTGTCGCTGGTGCGGTCTCAGACACCAGTGGTTTGCCACAAGATCGGAA12400.10956163858266116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)