FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442336
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC854615.925179708472922Illumina Single End PCR Primer 1 (100% over 50bp)
TCATGATCCTACTCAGCAGTGGAGCGCTGGTACCCTCCTGGGGATGCAGG37090.25715228629112774No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGGGTGTAGATCTCGGTGGTCGCC29930.20751059392540988Illumina Single End PCR Primer 1 (98% over 50bp)
TGTGTCGCTGGTGCGGTCTCAGATTTCGTGTTTTGTTGCTGACACAAGAT27830.19295087968406807No Hit
TGTGTCGCTGGTGCGGTCTCACACCTTCAACTCCTGCTACACAAGATCGG26290.18227375590708406No Hit
CTCCTACCTGGCCAATGAGATCCTGTGGGGTCACCGCTTTGAGCCCGTGC23790.16494076276262953No Hit
CTCCTCACACGCCATGAACTGGGAAACCTGCAATACACACAGAGCATGGG18100.12549087036585096No Hit
TGTGTCGCTGGTGCGGTCTCACCGGGGTCACCTCCCACACAAGATCGGAA16710.11585372617753421No Hit
CTCGTGCTCTGGGGCCAAAGCCCAGTAAACCAAGTTAACAAACCAAGCCA15900.11023783639873094No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)