FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1485084
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTA519713.4995326863665626TruSeq Adapter, Index 2 (97% over 36bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG92560.6232644079392142No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT85380.5749169743933676No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA68950.4642835018086519No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT46660.31419098178958227No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA40060.26974905123211884No Hit
TGTGCTGATGGTAAGATGACAGTTATGAGACCGCACCAGCGACACAAGAT37810.254598393087529No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATATCGTA30430.2049042343732745TruSeq Adapter, Index 2 (97% over 36bp)
TGTGCTGCTTGTCTGCCTCATGAGACCGCACCAGCGACACAAGATCGGAA26380.17763304971301289No Hit
TGTGACGAAATACTGGTTTTACCGCTGAGACCGCACCAGCGACACAAGAT22610.1522472802885224No Hit
TGTGTTGATTGAGGCAAAATATATTTGAGACCGCACCAGCGACACAAGAT22190.14941915743486564No Hit
TGTGTGGCCCGAGTAGGGAGTGAGACCGCACCAGCGACACAAGATCGGAA22020.14827444104171886No Hit
TGTGTCAAGTGGAAACAAAAACAAATGAGACCGCACCAGCGACACAAGAT18760.12632282079666873No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)