FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314826
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTA327192.4884661544569395TruSeq Adapter, Index 1 (97% over 37bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTA33920.2579809039370989TruSeq Adapter, Index 1 (97% over 37bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTA21120.1606296194325333TruSeq Adapter, Index 1 (97% over 37bp)
AGATGACCAAGACGAAGAAGATCATGTATGTTTTCCCGGCTGCTCGTAAA20330.15462121984201713No Hit
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATCTCGTA19790.15051421252698077TruSeq Adapter, Index 1 (97% over 37bp)
ACATGATAAATGGGCCTGTGACAAGGTAGGGATTATCATCCAACTGACAA19740.1501339340718848No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCATTCCATATCGTA18090.13758474505371812TruSeq Adapter, Index 1 (97% over 37bp)
TCATGGTCTCTGCTTACTACTTGATTCTCTCCTGCATGTTTTTGATGCCT17570.13362984912072015No Hit
CCGTGGGGCTGGAAGTCCTGAGAATAGGAATGATGGACATTTATCTGGTC17140.13035945440689492No Hit
GTATGACAATGGACAGTGCTGCCTGAGGGCTGCAGGTGCATGGCTGCGAA16960.12899045196854944No Hit
CTGGGGGACGAGGCCATGGAGCGCTTCCGCGAGGATGAGGGCTTCATTAA16080.12229755115886057No Hit
TGGTGTGTTCGTTCATGTCAACCATACACCCCACGACATCGCCTGCTTGC15930.12115671579357268No Hit
GGATGGAGACAATGAAACACGCCCAGCCATTCCAGTATTCAGTAGGGGGG15700.11940743490013127No Hit
AAATGGTGAGGCCCCAAAATAGACCAGTCCCGGGTCAGATAGTCGAAATT15280.11621309587732521No Hit
CAATGAGCAAATCAAACATGTGCTGGACTAACCTGGTTCCCTAACTGCCA15040.1143877592928646No Hit
CTCGTTCTTGTAGCAGAAGGAGTTGGCCCTGGGCAGCAGGAACTTGTTCT14920.1134750910006343No Hit
CTCGTTCTCATAGCAGAAGGAGTTGGCACTGGGCAGCAGGAACTTGTTCT14870.11309481254553834No Hit
CTATGGGTTACCTTCTCTTTGGGACCGATGGGTGCTGGGGAGGATCCCCC14620.11119342027005855No Hit
ACATGTCTGATGATCACGTCTGCACACTGAGTGAGCAGGGTTGGGGTGTT14120.10739063571909896No Hit
CTTTGTACGTTCCCTTTCACGAAAAAGAACCACCGTGAGCAACGTCCGCG13990.10640191173584945No Hit
CTCTGGTGTTTTTTAACCAGCCAGAGGCTCGTGCCACCACTTTTACCCAG13860.10541318775259996No Hit
CTGGGTCCAACAAAAGCACTGCCAGTAGCAAATCAGGGGATGGGAAGACC13720.10434840807833126No Hit
GTATGTAATGTGTGTGAGCATGTATGTATGTGGGAGCATGTGTAATCATG13610.10351179547712017No Hit
CTCGTTCTCGTAGCAGAAGGAGTTGGCGCTGGGCAGCAGGAACTTGTTCT13470.10244701580285147No Hit
TCATGGGCACTTTCACCCTCTGCTGGCTGCCCTTCTTCATCGTTAACATT13400.10191462596571713No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)