FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433087
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACTGGAATCTCGTA250531.7481841646738823TruSeq Adapter, Index 23 (97% over 38bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG33810.2359242669844887No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA21900.1528169608683911No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT18100.12630077587752872No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA17170.11981128849818609No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)