FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226199
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATCTCGTA1267745.694639158493917TruSeq Adapter, Index 1 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT45030.2022730223129199No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG36500.1639565914817139No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA29150.13094067511484822No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCACCTCATATCGTA28260.1269428294595407TruSeq Adapter, Index 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)