FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493609
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTA20870513.973201821895826TruSeq Adapter, Index 22 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTA75390.50475057394539TruSeq Adapter, Index 22 (97% over 38bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT21270.14240674768296122No Hit
TGTGTCGCTGGTGCGGTCTCATTGGAGGGGTCTCTGCCACAAGATCGGAA16200.1084621209432991No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA15570.1042441495732819No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)