FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001809333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090769
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTA192221.7622429680344787TruSeq Adapter, Index 10 (97% over 37bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTA19170.1757475689169751TruSeq Adapter, Index 10 (97% over 37bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG14880.13641751828297283No Hit
AGATGACCAAGACGAAGAAGATCATGTATGTTTTCCCGGCTGCTCGTAAA13630.12495771331968548No Hit
ACATGATAAATGGGCCTGTGACAAGGTAGGGATTATCATCCAACTGACAA13570.12440764268144767No Hit
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTA13010.11927365005789493TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATATCGTA12370.11340622991669179TruSeq Adapter, Index 10 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT12330.1130395161578666No Hit
GGATGGAGACAATGAAACACGCCCAGCCATTCCAGTATTCAGTAGGGGGG11520.1056135625416564No Hit
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTA11040.10121299743575404TruSeq Adapter, Index 7 (97% over 36bp)
GTATGACAATGGACAGTGCTGCCTGAGGGCTGCAGGTGCATGGCTGCGAA10910.10002117771957215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)