FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001809353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1785533
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTA19606710.980866777595262TruSeq Adapter, Index 6 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATATCGTA61130.34236275666705684TruSeq Adapter, Index 6 (97% over 37bp)
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT51760.2898854291687692No Hit
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG40830.2286712147017165No Hit
TGTGCCGAGGCCAGTTACCGTGAGACCGCACCAGCGACACAAGATCGGAA24810.13895010621478293No Hit
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA23950.1341336172448227No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT18380.10293845031147562No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)