FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001809359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001809359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295599
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTA772845.965117293236564TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTAGCAGGAGTTGAAGGTGTGAGACCGCACCAGCGACACAAGATCGG43110.33274184373405663No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATATCGTA34450.26590017435950475TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTGGGAGGTGACCCCGGTGAGACCGCACCAGCGACACAAGATCGGAA30070.23209341779362289No Hit
TGTGTCGAAGTAATCATTGCCTATCTGAGACCGCACCAGCGACACAAGAT24390.1882526923839861No Hit
TGTGATGCTGAGTTGGGTGCTGAGACCGCACCAGCGACACAAGATCGGAA21190.1635536921532048No Hit
TGTGTCAGCAACAAAACACGAAATCTGAGACCGCACCAGCGACACAAGAT16080.12411247615967595No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)